Literature summary for 4.1.99.3 extracted from

Li, C.; Ma, L.; Mou, S.; Wang, Y.; Zheng, Z.; Liu, F.; Qi, X.; An, M.; Chen, H.; Miao, J.
Cyclobutane pyrimidine dimers photolyase from extremophilic microalga remarkable UVB resistance and efficient DNA damage repair (2015), Mutat. Res., 773, 37-42 .

Search for more literature for 4.1.99.3

Cloned(Commentary)

Cloned (Comment)	Organism
gene phr2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, functional recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), DNA photolyase increases photo-repair more than 116fold in Escherichia coli repair-deficient strain SY2 under 0.1 mw/cm2UVB radiation	Chlamydomonas sp. ICE-L

Organism

Organism	UniProt	Comment	Textmining
Chlamydomonas sp. ICE-L	M4T971	isolated from the floating ice near the Zhongshan Research Station of Antarctica (69°S, 77°E)	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography. After purification in vitro, the cofactor FAD in photolyase is oxidized into the neutral radical form under aerobic conditions and the enzyme loses its repair function. Therefore, FAD is added to the photolyase	Chlamydomonas sp. ICE-L

Synonyms

Synonyms	Comment	Organism
class II CPD-photolyase	-	Chlamydomonas sp. ICE-L
CPD photolyase	-	Chlamydomonas sp. ICE-L
cyclobutane pyrimidine dimers photolyase	-	Chlamydomonas sp. ICE-L
DNA photolyase	-	Chlamydomonas sp. ICE-L
PHR2	-	Chlamydomonas sp. ICE-L

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	in vitro assay at room temperature	Chlamydomonas sp. ICE-L
37	-	in vivo assay	Chlamydomonas sp. ICE-L

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Chlamydomonas sp. ICE-L

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Release 2023.1 (January 2023)